PKC alpha/PRKCA Antibody orb234364 from Biorbyt

Product Specs
ItemPKC alpha/PRKCA Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb234364
Quantity10 µg, 100 µg
ApplicationsIHC, WB
Conjugate/TagUnconjugated
FormatLyophilized
ReactivityHuman, Mouse, Rat
TargetPKC alpha/PRKCA
IsotypeRabbit IgG
HostRabbit
ImmunogenE.coli-derived human PKC alpha recombinant protein (Position: M153-L342). Human PKC alpha shares 97% and 99% amino acid (aa) sequence identity with mouse and rat PKC alpha, respectively
NCBI Full Gene Nameprotein kinase C alpha
NCBI Gene AliasesAAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA
ClonalityPolyclonal
Molecular Weight76750 MW
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles

Specifications/Features

Application Notes: WB: The detection limit for PKC alpha is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Application Dilutions: Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat

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